This final report provides the background information and methodology employed to complete TCRP Project F-16a, Building a Sustainable Workforce in Public Transportation – A Systems Approach.    The Guidebook provides practical recommendations to public transportation stakeholders that are geared to help transit organizations build a more sustainable workforce and better orient themselves as an “employer of choice” among the current labor market and non-traditional candidates.  These recommendations are presented across a series of four modules which may be used independently by transit leaders or combined to form the fully integrated Guidebook. The modules address the following four areas: strategies for recruitment, retention, training and development, and professional capacity building (Module 1); metrics to evaluate the effectiveness of human resource practices adopted or contemplated (Module 2); reflections and strategies that pertain to image management (Module 3); and a framework for an ongoing benchmarking process (Module 4). Information across the modules is in the form of example successful programs, state-of-the-art initiatives, industry effective practices, and directions to implement and measure those practices. The Guidebook intends to serve all facets of the public transportation industry including large, medium, and small; urban and rural; rail and bus; public, private-for-profit, and private-nonprofit systems and vendors.